TAB1 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 67020-1-Ig (same clone as 67020-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67020-1-Ig (same clone as 67020-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67020-1-Ig (same clone as 67020-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67020-1-Ig (same clone as 67020-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67020-1-Ig (same clone as 67020-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67020-1-Ig (same clone as 67020-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67020-1-Ig (same clone as 67020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67020-1-PBS in a different storage buffer formulation.

TAB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67020-1-PBS

Clone No.1E10D12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MAP3K7IP1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAK1 binding protein 1, TAK1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

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Product Information

67020-1-PBS targets TAB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6590

  Product name: Recombinant human MAP3K7IP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-367 aa of BC050554

  Sequence: MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLLNNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGIICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPRHEDMTLLVRNFGY

• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC050554
• Gene Symbol: TAB1
• Gene ID (NCBI): 10454
• RRID: AB_2882336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15750
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.