TACC3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84940-5-RR (same clone as 84940-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84940-5-RR (TACC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84940-5-RR (same clone as 84940-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84940-5-RR (TACC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84940-5-RR (same clone as 84940-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC3 antibody (84940-5-RR, Clone: 242527A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84940-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84940-5-RR against Human TACC3 were performed. The affinity constant is 2.98 nM.

TACC3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84940-5-PBS

Clone No.242527A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242527A12, ERIC 1, ERIC1, ERIC-1, Maskin

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Product Information

84940-5-PBS targets TACC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag22516

  Product name: Recombinant human TACC3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 141-330 aa of BC111771

  Sequence: SSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGYPAGVGTPVPADSTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASS

• Full Name: transforming, acidic coiled-coil containing protein 3
• Calculated Molecular Weight: 838 aa, 91 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC111771
• Gene Symbol: TACC3
• Gene ID (NCBI): 10460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y6A5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.