TACC3 Recombinant antibody

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  WB analysis using 84940-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84940-5-RR (TACC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84940-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84940-5-RR against Human TACC3 were performed. The affinity constant is 2.98 nM.

TACC3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84940-5-RR

Clone No.242527A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TACC 3, Maskin, ERIC-1, ERIC1, ERIC 1

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Tested Applications

• Positive WB detected in: HeLa cells, SKOV-3 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84940-5-RR targets TACC3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TACC3 fusion protein Ag22516
• Full Name: transforming, acidic coiled-coil containing protein 3
• Calculated Molecular Weight: 838 aa, 91 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC111771
• Gene Symbol: TACC3
• Gene ID (NCBI): 10460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y6A5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.