TACR2 Polyclonal antibody

TACR2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25270-1-AP

Synonyms

Neurokinin A receptor, NK 2 receptor, NK 2R, NK2R, NKNAR, SKR, Substance K receptor, TAC2R, tachykinin receptor 2, TACR2

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Validation Data Gallery

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  WB analysis of HeLa using 25270-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25270-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25270-1-AP (TACR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 25270-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug TACR2 antibody (25270-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive FC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

25270-1-AP targets TACR2 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tachykinin receptor 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM_001057
• Gene Symbol: TACR2
• Gene ID (NCBI): 6865
• RRID: AB_2879998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tachykinins represent a family of peptide neurotransmitters including substance P, neurokinin A and neurokinin B, which act through the stimulation of tachykinin NK1, NK2 or NK3 receptors. NK2, also known as TACR2 (tachykinin receptor 2), is a receptor for the tachykinin neurokinin A (neuropeptide substance K). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; TACR2 is associated with the immune microenvironment and inhibits migration and proliferation via the Wnt/β-catenin signaling pathway in prostate cancer.; Authors - Wang Jianfeng
rat	IHC	Mol Med Rep; Occurrence of substance P and neurokinin receptors during the early phase of spinal fusion.; Authors - Suiyi Wu