TADA1L Polyclonal antibody

TADA1L Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20337-1-AP

Synonyms

KIAA0764, RP1 9E21.4, SPT3 associated factor 42, STAF42, TADA1, TADA1L, Transcriptional adapter 1

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Validation Data Gallery

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  WB analysis of mouse testis using 20337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20337-1-AP (TADA1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20337-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20337-1-AP (TADA1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 20337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20337-1-AP (TADA1L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20337-1-AP

  Immunofluorescent analysis of HepG2 cells using 20337-1-AP (TADA1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, NIH/3T3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20337-1-AP is TADA1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TADA1L fusion protein Ag14118
• Full Name: transcriptional adaptor 1 (HFI1 homolog, yeast)-like
• Calculated Molecular Weight: 335 aa, 37 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC015401
• Gene Symbol: TADA1L
• Gene ID (NCBI): 117143
• RRID: AB_2878675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.The MW of this protein is 37 kDa, and Catalog#20337-1-AP specially recognises the 37 kDa protein.