• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TAF12 Monoclonal antibody

TAF12 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4E12D2

Cat no : 67621-1-Ig

Synonyms

TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TAF12 Monoclonal antibody (67621-1-Ig)

    WB analysis using 67621-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67621-1-Ig (TAF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67621-1-Ig targets TAF12 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TAF12 fusion protein Ag16597
Full Name TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC011986
Gene Symbol TAF12
Gene ID (NCBI) 6883
RRIDAB_2882823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation

Protocols

Product Specific Protocols
WB protocol for TAF12 antibody 67621-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols