Multi-rAb® TAGLN Multi-Recombinant antibody

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  WB analysis using RMX00045

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00045 (TAGLN antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using RMX00045

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00045 (TAGLN antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using RMX00045

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using RMX00045 (TAGLN antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using RMX00045

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using RMX00045 (TAGLN antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using RMX00045

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using TAGLN antibody (RMX00045) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAGLN Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. RMX00045

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SM22, transgelin, 22 kDa actin-binding protein, Protein WS3-10, SM22-a

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue
• Positive IHC detected in: mouse heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00045 targets TAGLN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: transgelin
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC004927
• Gene Symbol: transgelin/SM22
• Gene ID (NCBI): 6876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q01995
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can cross-react with other two transgenes based on the sequence similarity.