Transgelin 2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Transgelin 2 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2E5G1

Cat no : 60044-1-Ig

Synonyms

SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2

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Validation Data Gallery

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  WB analysis of K-562 using 60044-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60044-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60044-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60044-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 60044-1-Ig

  IP Result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug.

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  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60044-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K562 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

60044-1-Ig targets Transgelin 2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transgelin 2 fusion protein Ag0337
• Full Name: transgelin 2
• Calculated molecular weight: 199 aa, 22 kDa
• Observed molecular weight: 22 kDa
• GenBank accession number: BC002616
• Gene symbol: TAGLN2
• Gene ID (NCBI): 8407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.; Authors - Liu Yang ; KD Validated