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  • KD/KO Validated

Transgelin 2 Monoclonal antibody

Transgelin 2 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse







Cat no : 60044-1-Ig


SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2

Tested Applications

Positive WB detected inK562 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human colon tissue, human colon cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60044-1-Ig targets Transgelin 2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337
Full Name transgelin 2
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002616
Gene Symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.


Product Specific Protocols
WB protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
IHC protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
IF protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
IP protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Med Rep

Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.

Authors - Liu Yang
  • KD Validated

Nat Commun

Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease

Authors - Xingyu Zhang

Cell Signal

TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells

Authors - Yongjia Gao