TAL1 Polyclonal antibody

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  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55317-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 55317-1-AP

  IP result of anti-TAL1 (IP:55317-1-AP, 4ug; Detection:55317-1-AP 1:1000) with Raji cells lysate 2000ug.

TAL1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 55317-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IP, ELISA

Synonyms

bHLHa17, SCL, Stem cell protein, tal 1, TAL1, TCL5

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

55317-1-AP targets TAL1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: T-cell acute lymphocytic leukemia 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_003189
• Gene Symbol: TAL1
• Gene ID (NCBI): 6886
• RRID: AB_2881306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17542
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

T-cell acute lymphocytic leukemia protein 1 (TAL1) also named Stem cell protein,T-cell leukemia/lymphoma protein 5.It is specifically expressed in leukemic stem cell,implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, serving as a positive regulator of erythroid differentiation. The 38kd, 44kd bands recognized by antibody are the phosphorylated forms of TAL1 and it's isoforms.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
zebrafish	WB	Development; BF170 hydrochloride enhances the emergence of hematopoietic stem and progenitor cells; Authors - WenYe Liu
human	WB	Pharmaceuticals (Basel); Caulis Polygoni Multiflori Accelerates Megakaryopoiesis and Thrombopoiesis via Activating PI3K/Akt and MEK/ERK Signaling Pathways; Authors - Xin Yang
		Nat Immunol; Tumor cell-released kynurenine biases MEP differentiation into megakaryocytes in individuals with cancer by activating AhR-RUNX1; Authors - Li Zhou
zebrafish	WB	Adv Sci (Weinh); Nap1L4a Cooperates with Scl/Klf1 to Recruit H2A.Z in Mediating Interactions Among Cis-Regulatory Elements and Transcription Required for Primitive Erythropoiesis in Zebrafish.; Authors - JiaHao Shi
mouse	WB	Sci Adv; Nat10-mediated ac4C epitranscriptomics orchestrates hematopoietic stem/progenitor cell fate determination via translation control.; Authors - Feng Huang
human	IF	Mol Cell; Alu repeat-containing RNAs spatially organize actively transcribed genomic regions around nuclear speckles.; Authors - Siqi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (12-19-2022)

TAl1 polyclonal worked well with transfected lysate by WB at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat and Karpas45 cells