TAL1 Polyclonal antibody

TAL1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 55317-1-AP

Synonyms

bHLHa17, SCL, Stem cell protein, tal 1, TAL1, TCL5

---

Validation Data Gallery

• 

  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 55317-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55317-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55317-1-AP (TAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 55317-1-AP

  IP result of anti-TAL1 (IP:55317-1-AP, 4ug; Detection:55317-1-AP 1:1000) with Raji cells lysate 2000ug.

---

Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55317-1-AP targets TAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: T-cell acute lymphocytic leukemia 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_003189
• Gene Symbol: TAL1
• Gene ID (NCBI): 6886
• RRID: AB_2881306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

T-cell acute lymphocytic leukemia protein 1 (TAL1) also named Stem cell protein,T-cell leukemia/lymphoma protein 5.It is specifically expressed in leukemic stem cell,implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, serving as a positive regulator of erythroid differentiation. The 38kd, 44kd bands recognized by antibody are the phosphorylated forms of TAL1 and it's isoforms.

Publications

Species	Application	Title
human	WB	Pharmaceuticals (Basel); Caulis Polygoni Multiflori Accelerates Megakaryopoiesis and Thrombopoiesis via Activating PI3K/Akt and MEK/ERK Signaling Pathways; Authors - Xin Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Wojciech (Verified Customer) (12-19-2022)

TAl1 polyclonal worked well with transfected lysate by WB at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat and Karpas45 cells