TAP1 Recombinant antibody

TAP1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 85766-4-RR
Clone No.250037E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HCT 116 cells, THP-1 cells, HepG2 cells, mouse spleen tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85766-4-RR targets TAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TAP1 fusion protein Ag1619 Predict reactive species
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC014081
Gene Symbol TAP1
Gene ID (NCBI) 6890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03518
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

Protocols

Product Specific Protocols
WB protocol for TAP1 antibody 85766-4-RRDownload protocol
IP protocol for TAP1 antibody 85766-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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