TBCC Monoclonal antibody

TBCC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B7D9

Cat no : 68371-1-Ig

Synonyms

CFC, TBCC, tubulin folding cofactor C, Tubulin specific chaperone C

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Validation Data Gallery

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  WB analysis using 68371-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of Calu-3 using 68371-1-Ig

  Calu-3 cells were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-1376 cells, Calu-3 cells, HeLa cells, MDA-MB-231 cells, MDA-MB-468 cells, HepG2 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68371-1-Ig targets TBCC in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBCC fusion protein Ag8962
• Full Name: tubulin folding cofactor C
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC020170
• Gene Symbol: TBCC
• Gene ID (NCBI): 6903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).