TBCCD1 Polyclonal antibody, PBS Only

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• TBCCD1 Polyclonal antibody, PBS Only (21429-1-PBS)

• 

  WB analysis using 21429-1-AP (same clone as 21429-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21429-1-AP (TBCCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21429-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 21429-1-AP (same clone as 21429-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBCCD1 antibody (21429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21429-1-PBS in a different storage buffer formulation.

TBCCD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 21429-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TBCC domain containing 1

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Product Information

21429-1-PBS targets TBCCD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16032

  Product name: Recombinant human TBCCD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 208-557 aa of BC025748

  Sequence: AVVALSFLIEGTISRARKIYPLHELALWQPLHADSGFSKISKTFSFYKLETWLRSCLTGNPFGTSACLKSGKKLAWAHQVEGTTKRAKIACNTHVAPRMHRLVVMSQVYKQTLAKSSDTLAGAHVKIHRCNESFIYLLSPLRSVTIEKCRNSIFVLGPVGTTLHLHSCDNVKVIAVCHRLSISSTTGCIFHVLTPTRPLILSGNQTVTFAPFHTHYPMLEDHMARTGLATVPNYWDNPMVVCRENSDTRVFQLLPPCEFYVFIIPFEMEGDTTEIPGGLPSVYQKALGQREQKIQIWQKTVKEAHLTKDQRKQFQVLVENKFYEWLINTGHRQQLDSLVPPAAGSKQAAG

• Full Name: TBCC domain containing 1
• Calculated Molecular Weight: 557 aa, 64 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC025748
• Gene Symbol: TBCCD1
• Gene ID (NCBI): 55171
• RRID: AB_3742033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9NVR7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TBCC-domain containing 1 (TBCCD1) belongs to the tubulin-binding cofactor C (TBCC) family, which is a new human pericentriolar matrix component (PMID: 24101722). TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization (PMID: 20168327).