TBCE Polyclonal antibody

• Home
• Products
• Primary Antibodies
• TBCE Polyclonal antibody (12021-1-AP)

• Featured Product
• KD/KO Validated

TBCE Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12021-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

HRD, KCS, KCS1, pac2, Tubulin-folding cofactor E

• 

  WB analysis using 12021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12021-1-AP (TBCE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12021-1-AP

  WB result of TBCE antibody (12021-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBCE transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 12021-1-AP

  WB result of TBCE antibody (12021-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBCE transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 12021-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12021-1-AP (TBCE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for TBCE in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12021-1-AP targets TBCE in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2650

  Product name: Recombinant human TBCE protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-330 aa of BC008654

  Sequence: MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEK

• Full Name: tubulin folding cofactor E
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC008654
• Gene Symbol: TBCE
• Gene ID (NCBI): 6905
• RRID: AB_2271440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15813
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TBCE Polyclonal antibody (Cat# 12021-1-AP) has 3 citations published in Cancer Research, Experimental Neurology, and Histochemistry and Cell Biology. Research fields include immune therapy in chronic myelogenous leukemia (CML), microtubule disturbance and cochlear outer hair cell degeneration associated with progressive hearing loss in pmn/pmn mice, and spatio-temporal distribution of tubulin-binding cofactors with posttranslational modifications of tubulin in the cochlea. Applications cited include WB, IHC, and IF in human and mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; Efficacious immune therapy in chronic myelogenous leukemia (CML) recognizes antigens that are expressed on CML progenitor cells.; Authors - Biernacki Melinda A MA
mouse	IHC	Exp Neurol; Mutation of the TBCE gene causes disturbance of microtubules in the auditory nerve and cochlear outer hair cell degeneration accompanied by progressive hearing loss in the pmn/pmn mouse.; Authors - Rak Kristen K
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens