Tested Applications
| Positive WB detected in | HEK-293 cells, U2OS cells, HepG2 cells, Jurkat cells, LNCaP cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67211-1-Ig targets TBK1 in WB, IF, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28894 Product name: Recombinant human TBK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 316-576 aa of BC034950 Sequence: LQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA Predict reactive species |
| Full Name | TANK-binding kinase 1 |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC034950 |
| Gene Symbol | TBK1 |
| Gene ID (NCBI) | 29110 |
| RRID | AB_2882504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UHD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBK1 antibody 67211-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBK1 Monoclonal antibody (Cat# 67211-1-Ig) has 63 citations. These appear in journals including Nature Cancer, Cell Molecular Immunology, Autophagy, ACS Nano, Advanced Science, Cell Reports, Cell Death & Differentiation, Journal of Neuroinflammation, Frontiers in Immunology, Journal of Virology, and Veterinary Research. Research fields span the cGAS-STING pathway, antiviral innate immunity, tumor immunotherapy, neuroinflammation, fibrosis, sepsis, and various viral infections, with most studies using Western blot.
| Species | Application | Title |
|---|---|---|
Nat Cancer A micropeptide encoded by the lncRNA USP30-AS1 promotes tumor growth by attenuating cGAS-STING-type I IFN signaling in macrophages | ||
Cell Mol Immunol Dynamic regulation of TBK1 lactylation shapes antiviral immune responses. | ||
Autophagy RNF144A inhibits autophagy by targeting BECN1 for degradation during L. monocytogenes infection | ||
ACS Nano Nanodrug Modulates Premetastatic Niche and Suppresses Metastatic Lung Adenocarcinoma via Programmed Cell Death Ligand 1 Blockade and STING Pathway Activation | ||
ACS Nano Biomimetic Nanoregulators Mediated Tyrosine Hydroxylase mRNA and Stimulator of Interferon Genes Antagonist Codelivery for Synergistic Therapy on Parkinson's Disease. |
Reviews
What customers say
The single review for this TBK1 antibody (rated 5/5) highlights excellent performance in Western Blot. The user tested it on 40-day-old retinal organoids derived from human iPSCs at a 1:1000 dilution, with 3% fish gelatin blocking and a 1:2000 secondary. They described the result as remarkably clean with no obvious background bands and expressed strong satisfaction, stating it would become their go-to TBK1 antibody going forward.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Karina (Verified Customer) (03-10-2026) | I'm very pleased with this antibody. It was remarkably clean, with no obvious background bands. This will be my go-to TBK1 antibody in the future. Blocked with 3% fish gelatin, used secondary at 1:2000.
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