TBK1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TBK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D7B1

Cat no : 67211-1-PBS

Synonyms

FLJ11330, NAK, NF kappa B activating kinase, T2K, TANK binding kinase 1, TBK1

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Validation Data Gallery

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  WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 67211-1-Ig (same clone as 67211-1-PBS)

  U2OS (WT and TBK1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 67211-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67211-1-PBS targets TBK1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBK1 fusion protein Ag28894
• Full Name: TANK-binding kinase 1
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC034950
• Gene Symbol: TBK1
• Gene ID (NCBI): 29110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.