TBX2 Polyclonal antibody

• 

  WB analysis of human placenta using 16930-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16930-1-AP (TBX2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 16930-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16930-1-AP (TBX2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16930-1-AP (TBX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16930-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16930-1-AP (TBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TBX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16930-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

T-box transcription factor TBX2, T-box protein 2, T-box 2, T box protein 2

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Tested Applications

• Positive WB detected in: human placenta tissue, BxPC-3 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

16930-1-AP targets TBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10393

  Product name: Recombinant human TBX2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC052566

  Sequence: MAYHPFHAPRPADFPMSAFLAAAQPSFFPALALPPGALAKPLPDPGLAGAAAAAAAAAAAAEAGLHVSALGPHPPAAHLRSLKSLEPEDEVEDDPKVTLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLPSLRLYEEHCKPERDGAESDASSCDPPPAREPPTSPGAAPSPLRLHRARAEEKSCAAD

• Full Name: T-box 2
• Calculated Molecular Weight: 702 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC052566
• Gene Symbol: TBX2
• Gene ID (NCBI): 6909
• RRID: AB_2200526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13207
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Chem; Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins.; Authors - Bianca Del B Sahm ; KD Validated
human	WB	Int J Mol Sci; Proteomics Analysis of the Polyomavirus DNA Replication Initiation Complex Reveals Novel Functional Phosphorylated Residues and Associated Proteins; Authors - Rama Dey-Rao