TBX4/5 Recombinant monoclonal antibody, PBS Only

• 

  IF Staining of U2OS using 84997-4-RR (same clone as 84997-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TBX5 antibody (84997-4-RR, Clone: 242565F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84997-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84997-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84997-4-RR against Human TBX4/5 were performed. The affinity constant is 37.3 nM.

TBX4/5 Uni-rAb^® Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 84997-4-PBS

Clone No.242565F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

TBX5, HOS, T box 5, T-box protein 5, T-box transcription factor TBX5

---

Product Information

84997-4-PBS targets TBX4/5 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3845

  Product name: Recombinant human TBX5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC027942

  Sequence: MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKE

• Full Name: T-box 5
• Calculated Molecular Weight: 518 aa, 57 kDa
• GenBank Accession Number: BC027942
• Gene Symbol: TBX5
• Gene ID (NCBI): 6910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99593
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.