TBX4/5 Recombinant antibody

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  IF Staining of U2OS using 84997-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TBX5 antibody (84997-4-RR, Clone: 242565F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84997-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84997-4-RR against Human TBX4/5 were performed. The affinity constant is 37.3 nM.

TBX4/5 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 84997-4-RR

Clone No.242565F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

TBX5, HOS, T box 5, T-box protein 5, T-box transcription factor TBX5

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84997-4-RR targets TBX4/5 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBX4/5 fusion protein Ag3845
• Full Name: T-box 5
• Calculated Molecular Weight: 518 aa, 57 kDa
• GenBank Accession Number: BC027942
• Gene Symbol: TBX5
• Gene ID (NCBI): 6910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99593
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.