TBXAS1 Polyclonal antibody

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  WB analysis of A549 using 11321-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11321-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11321-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11321-1-AP (TBXAS1 antibody) at dilution of 1:50 (under 10x lens).

TBXAS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11321-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CYP5, CYP5A1, Cytochrome P450 5A1, GHOSAL, TBXAS1, THAS, Thromboxane A synthase, TS, TXA synthase, TXAS, TXS

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Tested Applications

• Positive WB detected in: A549 cells, human colon tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

11321-1-AP targets TBXAS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1807

  Product name: Recombinant human TBXAS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 178-466 aa of BC014117

  Sequence: NKNRDELNGFFNKLIRNVIALRDQQAAEERRRDFLQMVLDARHSASPMGVQDFDIVRDVFSSTGCKPNPSRQHQPSPMARPLTVDEIVGQAFIFLIAGYEIITNTLSFATYLLATNPDCQEKLLREVDVFKEKHMAPEFCSLEEGLPYLDMVIAETLRMYPPAFRFTREAAQDCEVLGQRIPAGAVLEMAVGALHHDPEHWPSPETFNPERFTAEARQQHRPFTYLPFGAGPRSCLGVRLGLLEVKLTLLHVLHKFRFQACPETQVPLQLESKSALGPKNGVYIKIVSR

• Full Name: thromboxane A synthase 1 (platelet)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC014117
• Gene Symbol: TBXAS1
• Gene ID (NCBI): 6916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24557
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in humen uterus (PMID:1386258).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Hypertension; Reduced methylation of the thromboxane synthase gene is correlated with its increased vascular expression in preeclampsia.; Authors - Mousa Ahmad A AA
human	IF	Respir Res; Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity.; Authors - Shailendra R Singh
human	IHC,IF	Am J Physiol Lung Cell Mol Physiol; Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?; Authors - Singh Shailendra R SR
		Am J Physiol Renal Physiol; Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.; Authors - Katharina Mederle