• Featured Product
  • KD/KO Validated

TBXAS1 Monoclonal antibody

TBXAS1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F6G6

Cat no : 67371-1-Ig

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5



Tested Applications

Positive WB detected inhuman peripheral blood platelets, A549 cells, human placenta tissue, pig blood
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBXAS1 fusion protein Ag29177
Full Name thromboxane A synthase 1 (platelet)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC014117
Gene Symbol TBXAS1
Gene ID (NCBI) 6916
RRIDAB_2918487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).

Protocols

Product Specific Protocols
WB protocol for TBXAS1 antibody 67371-1-IgDownload protocol
IHC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
FC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Am J Physiol Renal Physiol

Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.

Authors - Katharina Mederle