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  • KD/KO Validated

TCF3 Monoclonal antibody

TCF3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D12E5

Cat no : 67140-1-Ig

Synonyms

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1



Tested Applications

Positive WB detected inHepG2 cells, NCI-H1299 cells, PC-3 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67140-1-Ig targets TCF3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TCF3 fusion protein Ag15734
Full Name transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated molecular weight 654 aa, 68 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC110580
Gene symbol TCF3
Gene ID (NCBI) 6929
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TCF3 antibody 67140-1-IgDownload protocol
IF protocol for TCF3 antibody 67140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Med Rep

Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.

Authors - Nian Zhou
  • KD Validated
humanWB

Reproduction

TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.

Authors - Xiao-Wei Wei
  • KD Validated