TCF7L1 Polyclonal antibody

TCF7L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14519-1-AP

Synonyms

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 14519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14519-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 14519-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of U2OS using 14519-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TCF7L1 antibody (14519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, JAR cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

14519-1-AP targets TCF7L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCF7L1 fusion protein Ag5983
• Full Name: transcription factor 7-like 1 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC058894
• Gene Symbol: TCF7L1
• Gene ID (NCBI): 83439
• RRID: AB_2287033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
zebrafish	IF	Nat Commun; Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling.; Authors - Kathleen Hübner
human	IF	Stem Cell Reports; Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta.; Authors - Sandra Haider
human	IHC	Oncogenesis; TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer.; Authors - Yu-Ching Wen
mouse	WB	iScience; Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity; Authors - Ting He
human	WB,IF	Endocrinology; Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility.; Authors - Gudrun Meinhardt