TCF7L1 Polyclonal antibody

TCF7L1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14519-1-AP

Synonyms

HMG box transcription factor 3, TCF 3, TCF3, TCF7L1, Transcription factor 7 like 1



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14519-1-AP targets TCF7L1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCF7L1 fusion protein Ag5983
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC058894
Gene symbol TCF7L1
Gene ID (NCBI) 83439
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

Protocols

Product Specific Protocols
WB protocol for TCF7L1 antibody 14519-1-APDownload protocol
IHC protocol for TCF7L1 antibody 14519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal

Authors - Jing Wang
zebrafishIF

Nat Commun

Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling.

Authors - Kathleen Hübner
humanIHC

Oncogenesis

TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer.

Authors - Yu-Ching Wen
humanWB,IF

Endocrinology

Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility.

Authors - Gudrun Meinhardt
ratWB

Korean J Physiol Pharmacol

The WNT/Ca2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria

Authors - Zhi-yu Li