TCF7L1/TCF7L2 Monoclonal antibody

TCF7L1/TCF7L2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B2D12

Cat no : 67652-1-Ig

Synonyms

HMG box transcription factor 3, HMG box transcription factor 4, TCF 3, TCF 4, TCF3, TCF4, TCF7L1, TCF7L2, Transcription factor 7 like 1

---

Validation Data Gallery

• 

  WB analysis using 67652-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67652-1-Ig (TCF7L1/TCF7L2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, A431 cells, NCCIT cells, JAR cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67652-1-Ig targets TCF7L1/TCF7L2 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TCF7L1/TCF7L2 fusion protein Ag28954
• Full Name: transcription factor 7-like 1 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC058894
• Gene Symbol: TCF7L1
• Gene ID (NCBI): 83439
• RRID: AB_2882851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.