Anti-Human TCR Vγ9Vδ2 Rabbit Recombinant Antibody

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• Anti-Human TCR Vγ9Vδ2 Rabbit Recombinant Antibody (98526-1-RR)

TCR Vγ9Vδ2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98526-1-RR Copied!

Clone No. 251678A1

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TCR gamma delta, Vγ9Vδ2

• 

  FC experiment of human PBMCs using 98526-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-TCR Vγ9Vδ2 Rabbit RecAb (98526-1-RR, Clone:251678A1), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98526-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-TCR Vγ9Vδ2 Rabbit RecAb (98526-1-RR, Clone:251678A1)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98526-1-RR targets TCR Vγ9Vδ2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: TCR Vγ9Vδ2
• RRID: AB_3746154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TCR Vγ9Vδ2 refers to a specific type of T cell receptor (TCR) found on a subset of gamma delta (γδ) T cells called Vγ9Vδ2 T cells. These cells are a major population of human γδ T cells in the blood and are characterized by the expression of a unique TCR that recognizes phosphoantigens (pAg). Vγ9Vδ2 T cell activation is tightly regulated by a variety of activating or inhibitory receptors which are specific for constitutively expressed or stress-modulated ligands (PMID: 20447835, 40040716). The most effective TCR Vg9Vd2-mediated cell activation relies on TCR interaction with BTN3A1, BTN3A2, and BTN2A1 molecules expressed by target cells (PMID: 25566259).