CoraLite® Plus 488 Anti-Mouse TCR Beta (H57-597)

TCR Beta Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65106
Clone No.H57-597

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-65106 targets TCR Beta in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Affinity purified TCR from mouse DO-11.10 cells Predict reactive species
Full Name T-cell receptor beta chain
Gene Symbol TCR beta
Gene ID (NCBI) 21577
RRIDAB_2883180
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TCR Beta antibody CL488-65106Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Sci Adv

Stimulator of interferon gene facilitates recruitment of effector CD8 T cells that drive neurofibromatosis type 1 nerve tumor initiation and maintenance

Authors - Jay Pundavela
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