CoraLite® Plus 488 Anti-Mouse TCR Beta (H57-597)

TCR Beta Monoclonal Antibody for IF, FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

H57-597

Cat no : CL488-65106

Synonyms

T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL488-65106

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse TCR beta (CL488-65106, Clone: H57-597) at dilution of 1:300.

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  FC experiment of C57BL/6 mouse splenocytes using CL488-65106

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse TCR Beta (CL488-65106, Clone: H57-597) or 0.25 ug CoraLite® Plus 488 Armenian Hamster IgG Isotype Control (PIP) (CL488-65210, Clone: PIP). Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:150-1:600

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65106 targets TCR Beta in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Affinity purified TCR from mouse DO-11.10 cells
• Full Name: T-cell receptor beta chain
• Gene Symbol: Tcrb
• Gene ID (NCBI): 21577
• RRID: AB_2883180
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).