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TDP-43 Recombinant antibody
TDP-43 Recombinant Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Human, mouse, rat
WB, IHC, FC,ELISA
Cat no : 80001-1-RR
Validation Data Gallery
|Positive WB detected in||HeLa cells, K-562 cells, Neuro-2a cells, C6 cells|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue, spinal cord slides from ALS patients, frontal cortex from FTLD-TDP type B|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:4000|
|Sample-dependent, check data in validation data gallery|
80001-1-RR targets TDP-43 in WB, IHC, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TDP-43 fusion protein Ag1231|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.
In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).
Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).
80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.
|Product Specific Protocols|
|WB protocol for TDP-43 antibody 80001-1-RR||Download protocol|
|IHC protocol for TDP-43 antibody 80001-1-RR||Download protocol|
|IF protocol for TDP-43 antibody 80001-1-RR||Download protocol|
|FC protocol for TDP-43 antibody 80001-1-RR||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (02-20-2020)
Good antibody. I have used it to stain both Ethanol and NBF fixed human Control and FTLD-TDP brain section and spinal cord from ALS cases by IHC. I have tested either citrate buffer or FA as antigen retrieval solutions, and in both conditions nicely detected the normal nuclear TDP-43 as well as cytoplasmic inclusions specially in spinal cord form ALS cases . The dilutions that worked better showing high specificity and low background ranged from 5,000-10,000.