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TDP-43 Recombinant antibody

TDP-43 Recombinant Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

11N20

Cat no : 80001-1-RR

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells, C6 cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, spinal cord slides from ALS patients, frontal cortex from FTLD-TDP type B
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
Sample-dependent, check data in validation data gallery

Product Information

80001-1-RR targets TDP-43 in WB, IHC, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TDP-43 fusion protein Ag1231
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene.In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176)Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).

80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 80001-1-RRDownload protocol
IHC protocol for TDP-43 antibody 80001-1-RRDownload protocol
IF protocol for TDP-43 antibody 80001-1-RRDownload protocol
FC protocol for TDP-43 antibody 80001-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (02-20-2020)

Good antibody. I have used it to stain both Ethanol and NBF fixed human Control and FTLD-TDP brain section and spinal cord from ALS cases by IHC. I have tested either citrate buffer or FA as antigen retrieval solutions, and in both conditions nicely detected the normal nuclear TDP-43 as well as cytoplasmic inclusions specially in spinal cord form ALS cases . The dilutions that worked better showing high specificity and low background ranged from 5,000-10,000.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:5,000 1: 10,000
  • Cell Tissue Type: human cortex and spinal cord
TDP-43 Antibody Immunohistochemistry, validation (1:5,000 1: 10,000 dilution) in human cortex and spinal cord (Cat no:80001-1-RR)