TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B1B12

Cat no : 66845-1-Ig

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

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Validation Data Gallery

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  WB analysis using 66845-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66845-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66845-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66845-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 66845-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 66845-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66845-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66845-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, JAR cells, NCCIT cells, LNCaP cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat testis tissue, mouse testis tissue, Rat brain
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66845-1-Ig targets TDRKH in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TDRKH fusion protein Ag4720
• Full Name: tudor and KH domain containing
• Calculated Molecular Weight: 606 aa, 67 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC032690
• Gene Symbol: TDRKH
• Gene ID (NCBI): 11022
• RRID: AB_2882185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.