Filter: AllWBIHCIF

WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
WB analysis of NCCIT using 66845-1-Ig (same clone as 66845-1-PBS)
NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 66845-1-Ig (same clone as 66845-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

TDRKH Monoclonal antibody, PBS Only

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66845-1-PBS

Clone No.2B1B12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66845-1-PBS targets TDRKH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity	Human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag4720 Product name: Recombinant human TDRKH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-374 aa of BC032690 Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR Predict reactive species
Full Name	tudor and KH domain containing
Calculated Molecular Weight	606 aa, 67 kDa
Observed Molecular Weight	67-70 kDa
GenBank Accession Number	BC032690
Gene Symbol	TDRKH
Gene ID (NCBI)	11022
RRID	AB_2882185
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9Y2W6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.