TDRKH Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66845-1-Ig (same clone as 66845-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66845-1-Ig (same clone as 66845-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51542-2

  Cytometric bead array sample test of MP51542-2, TDRKH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66845-1-PBS. Detection antibody: 66845-4-PBS.

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  Cytometric bead array standard curve of MP51542-2

  Cytometric bead array standard curve of MP51542-2, TDRKH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66845-1-PBS. Detection antibody: 66845-4-PBS. Standard:Ag4720. Range: 3.125-400 ng/mL.

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66845-1-PBS

Clone No.2B1B12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

TDRKH specific, TDRD2, tudor and KH domain containing, Tudor and KH domain-containing protein, Tudor domain-containing protein 2

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Product Information

66845-1-PBS targets TDRKH as part of a matched antibody pair:

MP51542-2: 66845-1-PBS capture and 66845-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4720

  Product name: Recombinant human TDRKH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-374 aa of BC032690

  Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR

• Full Name: tudor and KH domain containing
• Calculated Molecular Weight: 606 aa, 67 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC032690
• Gene Symbol: TDRKH
• Gene ID (NCBI): 11022
• RRID: AB_2882185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y2W6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.