TEAD3 Monoclonal antibody

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  WB analysis using 68464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of human placenta using 68464-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TEAD3 antibody (68464-1-Ig, Clone: 2E6A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TEAD3 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 68464-1-Ig

Clone No.2E6A12

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF-P, ELISA

Synonyms

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

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Tested Applications

• Positive WB detected in: HCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68464-1-Ig targets TEAD3 in WB, IF-P, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4407

  Product name: Recombinant human TEAD3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-324 aa of BC027877

  Sequence: MNLDQVSKDKALQSMASMSSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD

• Full Name: TEA domain family member 3
• Calculated Molecular Weight: 435 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC027877
• Gene Symbol: TEAD3
• Gene ID (NCBI): 7005
• RRID: AB_3085175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99594
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.