TEKT3 Polyclonal antibody

TEKT3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12959-1-AP

Synonyms

TEKT3, tektin 3

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Validation Data Gallery

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  WB analysis of mouse testis using 12959-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12959-1-AP (TEKT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12959-1-AP

  IP result of anti-TEKT3 (IP:12959-1-AP, 4ug; Detection:12959-1-AP 1:1000) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of human testis using 12959-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12959-1-AP (TEKT3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12959-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12959-1-AP (TEKT3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12959-1-AP targets TEKT3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEKT3 fusion protein Ag4080
• Full Name: tektin 3
• Calculated Molecular Weight: 490 aa, 57 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC031688
• Gene Symbol: TEKT3
• Gene ID (NCBI): 64518
• RRID: AB_2877898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP	Cell Mol Life Sci; Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella; Authors - Xin-Yan Geng
mouse	WB,IF	J Genet Genomics; Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice; Authors - Yaling Wang