TEX10 Polyclonal antibody

• Featured Product
• KD/KO Validated

TEX10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17372-1-AP

Synonyms

Testis-expressed protein 10, testis expressed 10, Nbla10363, L18

---

Validation Data Gallery

• 

  WB analysis of HeLa using 17372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17372-1-AP (TEX10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17372-1-AP

  WB result of TEX10 antibody (17372-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX10 transfected HeLa cells.

• 

  IHC staining of human brain using 17372-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17372-1-AP (TEX10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 17372-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17372-1-AP (TEX10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17372-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17372-1-AP (TEX10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

17372-1-AP targets TEX10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX10 fusion protein Ag11014
• Full Name: testis expressed 10
• Calculated Molecular Weight: 929 aa, 106 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC030652
• Gene Symbol: TEX10
• Gene ID (NCBI): 54881
• RRID: AB_2201871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX10 is a component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5. PELP1-TEX10-WDR18 complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation. And this complex is required for the maturation and nucleolar release of the large ribosomal subunit. (PMID:21326211)

Publications

Species	Application	Title
human	WB,IF	EMBO J; The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.; Authors - Finkbeiner Elisabeth E ; KD Validated
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB,IHC,IF	Cell Cycle; Tex10 is upregulated and promotes cancer stem cell properties and chemoresistance in hepatocellular carcinoma.; Authors - Xiaocong Xiang ; KD Validated
human	WB,IF	Mol Biol Cell; LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.; Authors - Castle Christopher D CD
human	WB	J Immunol Res; TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6; Authors - Sheng Luo ; KD Validated
human	WB,IHC	Oncol Rep; Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/β‑catenin pathway.; Authors - Xiaocong Xiang ; KD Validated