Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17372-1-AP targets TEX10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11014 Product name: Recombinant human TEX10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 336-757 aa of BC030652 Sequence: VPPQLATPVGNGIEREPLQVMQQVLNIISLLWKLSKQQDETHKLESWLRKNYLIDFKHHFMSRFPYVLKEITKHKGKEPNKSIKHCTVLSNNIDHLLLNLTLSDIMVSLANASTLQKDCSWIEMIRKFVTETLEDGSRLNSKQLNRLLGVSWRLMQIQPNREDTETLIKAVYTLYQQRGLILPVRTLLLKLFSKIYQTEELRSCRFRYRSKVLSRWLAGLPLQLAHLGSRNPELSTQLIDIIHTAAARANKELLKSLQATALRIYDPQEGAVVVLPADSQQRLVQLVYFLPSLPADLLSRLSRCCIMGRLSSSLAAMLIGILHMRSSFSGWKYSAKDWLMSDVDYFSFLFSTLTGFSKEELTWLQSLRGVPHVIQTQLSPVLLYLTDLDQFLHHWDVTEAVFHSLLVIPARSQNFDILQSAI Predict reactive species |
| Full Name | testis expressed 10 |
| Calculated Molecular Weight | 929 aa, 106 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC030652 |
| Gene Symbol | TEX10 |
| Gene ID (NCBI) | 54881 |
| RRID | AB_2201871 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEX10 is a component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5. PELP1-TEX10-WDR18 complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation. And this complex is required for the maturation and nucleolar release of the large ribosomal subunit. (PMID:21326211)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TEX10 antibody 17372-1-AP | Download protocol |
| WB protocol for TEX10 antibody 17372-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TEX10 antibody (Cat# 17372-1-AP) has 14 total citations published across 13 journals including Advanced Science, EMBO Journal, Molecular Cell Proteomics, Oncology Reports, Molecular Oncology, Cancer Research Communications, Cell Cycle, Molecular Carcinogenesis, Breast Cancer Research and Treatment, Journal of Immunology Research, Molecular Biology of the Cell, Biochemical and Biophysical Research Communications, and Bioengineered. Research fields span cancer biology (colorectal, esophageal, hepatocellular, breast, bladder, prostate, endometrial), ribosome biogenesis, SUMOylation, and chromatin regulation.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Targeting Tex10 Overcomes Oxaliplatin Resistance by Competitively Disrupting the Non-Canonical BAF Complex in Colorectal Cancer
| ||
EMBO J The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
| ||
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Oncol Rep Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/β‑catenin pathway.
| ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis | ||
