CoraLite®594-conjugated TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11D6

Cat no : CL594-67982

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

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Validation Data Gallery

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  IF Staining of mouse testis using CL594-67982

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

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  IF Staining of mouse testis using CL594-67982

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67982 targets TEX14 in IF applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TEX14 fusion protein Ag13143
• Full Name: testis expressed 14
• Calculated Molecular Weight: 957aa,107 kDa; 1497aa,168 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC040526
• Gene Symbol: TEX14
• Gene ID (NCBI): 56155
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.