TFAP2D Polyclonal antibody

TFAP2D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18835-1-AP

Synonyms

AP2 delta, TFAP2BL1, TFAP2D

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Validation Data Gallery

• 

  WB analysis of HeLa using 18835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18835-1-AP (TFAP2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18835-1-AP targets TFAP2D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transcription factor AP-2 delta (activating enhancer binding protein 2 delta)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC166653
• Gene Symbol: TFAP2D
• Gene ID (NCBI): 83741
• RRID: AB_2878562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAP2D, also named as TFAP2BL1, belongs to the AP-2 family. TFAP2D is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. It is highly expressed in adult thymus, prostate, small intestine, skeletal muscle, placenta, brain, and testis tissues.