TFAP4 Recombinant monoclonal antibody

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• TFAP4 Recombinant monoclonal antibody (83196-4-RR)

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• KD/KO Validated

TFAP4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83196-4-RR Copied!

Clone No. 240031D5

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ChIP, ELISA
For FC, select conjugated version.

Synonyms

240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41

• 

  WB analysis using 83196-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 83196-4-RR

  WB result of TFAP4 antibody (83196-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAP4 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 83196-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83196-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, A549 cells, NCI-H1299 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83196-4-RR targets TFAP4 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2633

  Product name: Recombinant human TFAP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC010576

  Sequence: MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

• Full Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010576
• Gene Symbol: TFAP4
• Gene ID (NCBI): 7023
• RRID: AB_3670886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01664
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Rabbit Recombinant anti-TFAP4 antibody (Cat# 83196-4-RR) has 3 total citations appearing in Cell Stem Cell, Scientific Reports, and Experimental Cell Research. The associated research fields include breast cancer metastasis and transcription factor-driven epigenetic remodeling, hearing acclimatization to high altitude in rats, and prostate cancer tumor progression involving macrophage M2 polarization through JAK2/STAT3 signaling activation.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Cell Stem Cell; Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis.; Authors - Xinxin Rao ; KO Validated
rat	IF	Sci Rep; Transcriptional mechanisms of hearing acclimatization to high altitude in rats.; Authors - Danzeng Awang
human	WB,IF	Exp Cell Res; TFAP4/DLGAP5 promotes tumor progression and macrophage M2 polarization in prostate cancer by activating the JAK2/STAT3 signaling.; Authors - Sen Li ; KD Validated