TFAP4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83196-4-RR (same clone as 83196-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 83196-4-RR (same clone as 83196-4-PBS)

  WB result of TFAP4 antibody (83196-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAP4 transfected HeLa cells. This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83196-4-RR (same clone as 83196-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83196-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83196-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

TFAP4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 83196-4-PBS

Clone No.240031D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41

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Product Information

83196-4-PBS targets TFAP4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2633

  Product name: Recombinant human TFAP4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-338 aa of BC010576

  Sequence: MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

• Full Name: transcription factor AP-4 (activating enhancer binding protein 4)
• Calculated Molecular Weight: 338 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010576
• Gene Symbol: TFAP4
• Gene ID (NCBI): 7023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01664
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.