TFB1M Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84957-4-RR (same clone as 84957-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84957-4-RR (TFB1M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 84957-4-RR (same clone as 84957-4-PBS)

  IP result of anti-TFB1M (IP:84957-4-RR, 4ug; Detection:84957-4-RR 1:1500) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84957-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84957-4-RR against Human TFB1M were performed. The affinity constant is 2.99 nM.

TFB1M Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 84957-4-PBS

Clone No.242524A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-

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Product Information

84957-4-PBS targets TFB1M in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9785

  Product name: Recombinant human TFB1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC017788

  Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

• Full Name: transcription factor B1, mitochondrial
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC017788
• Gene Symbol: TFB1M
• Gene ID (NCBI): 51106
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WVM0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.