TFB1M Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• TFB1M Recombinant monoclonal antibody, PBS Only (84957-4-PBS)

• 

  WB analysis using 84957-4-RR (same clone as 84957-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84957-4-RR (TFB1M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 84957-4-RR (same clone as 84957-4-PBS)

  IP result of anti-TFB1M (IP:84957-4-RR, 4ug; Detection:84957-4-RR 1:1500) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84957-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84957-4-RR against Human TFB1M were performed. The affinity constant is 2.99 nM.

TFB1M Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 84957-4-PBS

Clone No.242524A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-

---

Product Information

84957-4-PBS targets TFB1M in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9785

  Product name: Recombinant human TFB1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC017788

  Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

• Full Name: transcription factor B1, mitochondrial
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC017788
• Gene Symbol: TFB1M
• Gene ID (NCBI): 51106
• RRID: AB_3743884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WVM0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)