TFB1M Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• TFB1M Recombinant monoclonal antibody (84957-4-RR)

• 

  WB analysis using 84957-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84957-4-RR (TFB1M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 84957-4-RR

  IP result of anti-TFB1M (IP:84957-4-RR, 4ug; Detection:84957-4-RR 1:1500) with HepG2 cells lysate 1600 ug.

• 

  IHC staining of human colon cancer using 84957-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 84957-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84957-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84957-4-RR against Human TFB1M were performed. The affinity constant is 2.99 nM.

TFB1M Uni-rAb^® Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 84957-4-RR

Clone No.242524A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84957-4-RR targets TFB1M in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9785

  Product name: Recombinant human TFB1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-346 aa of BC017788

  Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

• Full Name: transcription factor B1, mitochondrial
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC017788
• Gene Symbol: TFB1M
• Gene ID (NCBI): 51106
• RRID: AB_3743884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WVM0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)