TFE3 Polyclonal antibody

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• TFE3 Polyclonal antibody (14480-1-AP)

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• KD/KO Validated

TFE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14480-1-AP Copied!

Publications (34)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

bHLHe33, Class E basic helix-loop-helix protein 33, RCCP2, TFE 3, TFEA

• 

  WB analysis of K-562 using 14480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 14480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 14480-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 14480-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 14480-1-AP

  IP result of anti-TFE3 (IP:14480-1-AP, 4ug; Detection:14480-1-AP 1:500) with K-562 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of K-562 using 14480-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TFE3 antibody (14480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14480-1-AP targets TFE3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5863

  Product name: Recombinant human TFE3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC026027

  Sequence: MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN

• Full Name: transcription factor binding to IGHM enhancer 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026027
• Gene Symbol: TFE3
• Gene ID (NCBI): 7030
• RRID: AB_2199587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19532
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Publications

What published studies show

Anti-TFE3 antibody (Cat# 14480-1-AP) has 34 citations across 27 journals including Autophagy, Nature Communications, PNAS, Cell Stem Cell, and Oncogene. Research fields span autophagy and lysosomal biogenesis, cancer (renal cell carcinoma, hepatocellular carcinoma, breast cancer, lung cancer), neurodegeneration (Huntington disease, frontotemporal dementia), stem cell biology, inflammation, lipid metabolism, and viral infections.

Automatically generated from the 34 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Stem Cell; Niche inflammatory signals control oscillating mammary regeneration and protect stem cells from cytotoxic stress; Authors - Chunye Liu
		Autophagy; TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ; Authors - Chang Woo Chae
human	WB,IF	Autophagy; FGF21 rejuvenates aged human adipose-derived mesenchymal stem cells via enhancement of TFE3-mediated autophagy flux.; Authors - Bei Song ; KD Validated
		Autophagy; IL17A disrupts autophagy-lysosomal function and lysosome reformation through the GSK3B-TFE3 signaling pathway in Huntington disease.; Authors - Kai-Po Chen
human	WB	Autophagy; Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.; Authors - Hongfeng Wang
human	WB	Autophagy; The human vault RNA enhances tumorigenesis and chemoresistance through the lysosome in hepatocellular carcinoma; Authors - Iolanda Ferro