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  • KD/KO Validated

TFE3 Polyclonal antibody

TFE3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14480-1-AP

Synonyms

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3



Tested Applications

Positive WB detected inK-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14480-1-AP targets TFE3 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFE3 fusion protein Ag5863
Full Name transcription factor binding to IGHM enhancer 3
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC026027
Gene symbol TFE3
Gene ID (NCBI) 7030
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Protocols

Product Specific Protocols
WB protocol for TFE3 antibody 14480-1-APDownload protocol
IHC protocol for TFE3 antibody 14480-1-APDownload protocol
IP protocol for TFE3 antibody 14480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Autophagy

Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.

Authors - Hongfeng Wang
humanWB,IF,IP

Autophagy

PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.

Authors - Bo Wang
  • KD Validated
mouseWB

Cell Discov

Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.

Authors - Yiting Jia
humanWB,IF

Bioorg Med Chem

Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.

Authors - Siwen Li
humanWB

Biochim Biophys Acta Gen Subj

Anti-cancer effect of marchantin C via inducing lung cancer cellular senescence associated with less secretory phenotype.

Authors - Xiu-Lei Zhang