TFE3 Polyclonal antibody

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  WB analysis of K-562 using 14480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14480-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14480-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14480-1-AP

  IP result of anti-TFE3 (IP:14480-1-AP, 4ug; Detection:14480-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TFE3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14480-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

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Tested Applications

• Positive WB detected in: K-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• ChIP: See 4 publications below

Product Information

14480-1-AP targets TFE3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5863

  Product name: Recombinant human TFE3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC026027

  Sequence: MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN

• Full Name: transcription factor binding to IGHM enhancer 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC026027
• Gene Symbol: TFE3
• Gene ID (NCBI): 7030
• RRID: AB_2199587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19532
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.; Authors - Hongfeng Wang
human	WB,IF,IP	Autophagy; PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.; Authors - Bo Wang ; KD Validated
mouse	WB	Cell Discov; Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.; Authors - Yiting Jia
human	ChIP	J Biol Chem; TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells; Authors - Suli Lv
human	WB,IF	Bioorg Med Chem; Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.; Authors - Siwen Li