TFIIE Beta Polyclonal antibody, PBS Only

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• TFIIE Beta Polyclonal antibody, PBS Only (11596-1-PBS)

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  WB analysis of K-562 using 11596-1-AP (same clone as 11596-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 11596-1-AP (same clone as 11596-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11596-1-AP (same clone as 11596-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11596-1-AP (TFIIE beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 11596-1-AP (same clone as 11596-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using TFIIE Beta antibody (11596-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11596-1-PBS in a different storage buffer formulation.

TFIIE Beta Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 11596-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

11596-1-PBS targets TFIIE Beta in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2136

  Product name: Recombinant human TFIIE beta protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3-291 aa of BC030572

  Sequence: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK

• Full Name: general transcription factor IIE, polypeptide 2, beta 34kDa
• Calculated Molecular Weight: 291 aa, 33 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC030572
• Gene Symbol: GTF2E2
• Gene ID (NCBI): 2961
• RRID: AB_2232722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29084
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).