TGFBI/BIGH3 Polyclonal antibody, PBS Only

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• TGFBI/BIGH3 Polyclonal antibody, PBS Only (10188-1-PBS)

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• KD/KO Validated

TGFBI/BIGH3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 10188-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

• 

  WB analysis using 10188-1-AP (same clone as 10188-1-PBS)

  Mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10188-1-AP (same clone as 10188-1-PBS)

  WB result of TGFBI / BIGH3 antibody (10188-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBI / BIGH3 transfected HeLa cells. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10188-1-AP (same clone as 10188-1-PBS)

  Untreated A549 cells and TGF beta 1 treated A549 cells were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI/BIGH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10188-1-AP (same clone as 10188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 10188-1-AP (same clone as 10188-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10188-1-AP (same clone as 10188-1-PBS)

  IP result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse eye using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 10188-1-AP (same clone as 10188-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells, untreated (left) or TGF-β-treated (right), using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human TGFBI / BIGH3 (10188-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

10188-1-PBS targets TGFBI/BIGH3 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0241

  Product name: Recombinant human BIGH3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 199-406 aa of BC000097

  Sequence: NIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSG

• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 683 aa, 75 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC000097
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• ENSEMBL Gene ID: ENSG00000120708
• RRID: AB_2202311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15582
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).