TH Recombinant monoclonal antibody

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• TH Recombinant monoclonal antibody (85929-5-RR)

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  WB analysis of PC-12 using 85929-5-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85929-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85929-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85929-5-RR (TH antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85929-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85929-5-RR (TH antibody) at dilution of 1:30000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85929-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85929-5-RR (TH antibody) at dilution of 1:30000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 85929-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 85929-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TH antibody (85929-5-RR, Clone: 250146D7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85929-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85929-5-RR against Human TH were performed. The affinity constant is 47.4 pM.

TH Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85929-5-RR

Clone No.250146D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

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Tested Applications

• Positive WB detected in: rat brain tissue, PC-12 cells, mouse brain tissue, fetal human brain tissue
• Positive IHC detected in: mouse brain tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1250-1:5000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85929-5-RR targets TH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag23034

  Product name: Recombinant human TH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC104967

  Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

• Full Name: tyrosine hydroxylase
• Calculated Molecular Weight: 528 aa, 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC104967
• Gene Symbol: TH
• Gene ID (NCBI): 7054
• RRID: AB_3744480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07101
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.