Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15208-1-AP targets THG1L in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7361 Product name: Recombinant human THG1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC001852 Sequence: MAKSKFEYVRDFEADDTCLAHCWVVVRLDGRNFHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQSDEYSFVFKRKTNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLYPPGFDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQQSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNELPMYRKGTVLIWQKVDEVMTKEIKLPTEMEGKKMAVTRTRTKPVPLHCDIIGDAFWKEHPEILDEDS Predict reactive species |
| Full Name | tRNA-histidine guanylyltransferase 1-like (S. cerevisiae) |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC001852 |
| Gene Symbol | THG1L |
| Gene ID (NCBI) | 54974 |
| RRID | AB_2303248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for THG1L antibody 15208-1-AP | Download protocol |
| IHC protocol for THG1L antibody 15208-1-AP | Download protocol |
| WB protocol for THG1L antibody 15208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The THG1L Polyclonal antibody (Cat# 15208-1-AP) has 1 total citation. It appears in Biochimica et Biophysica Acta (IF: 5), published in January 2017. The cited study investigated profibrotic IHG-1 complexes with HSPA5 and TRAP1 in mitochondria, placing the research in the fields of fibrosis, renal disease, and mitochondrial biology. The antibody was used for Western blot and immunoprecipitation in human samples.
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Profibrotic IHG-1 complexes with renal disease associated HSPA5 and TRAP1 in mitochondria. |
