THOC1 Recombinant antibody, PBS Only

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  WB analysis using 85642-5-RR (same clone as 85642-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85642-5-RR (THOC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85642-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85642-5-RR (same clone as 85642-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (85642-5-RR, Clone: 243161E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85642-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85642-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85642-5-RR against Human THOC1 were performed. The affinity constant is below 1 pM.

THOC1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85642-5-PBS

Clone No.243161E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1

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Product Information

85642-5-PBS targets THOC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THOC1 fusion protein Ag1352
• Full Name: THO complex 1
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC010381
• Gene Symbol: THOC1
• Gene ID (NCBI): 9984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FV9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy.