THOC1 Recombinant antibody

• 

  WB analysis using 85642-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85642-5-RR (THOC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 85642-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (85642-5-RR, Clone: 243161E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 85642-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85642-5-RR against Human THOC1 were performed. The affinity constant is below 1 pM.

THOC1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85642-5-RR

Clone No.243161E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Nuclear matrix protein p84, HPR1, p84N5, Tho 1, THO complex 1

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, U-87 MG cells, HeLa cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85642-5-RR targets THOC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THOC1 fusion protein Ag1352
• Full Name: THO complex 1
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC010381
• Gene Symbol: THOC1
• Gene ID (NCBI): 9984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy.