THOC6 Polyclonal antibody

THOC6 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15316-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

fSAP35, THO complex subunit 6 homolog, THOC6, WDR58

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIPIHC

WB analysis using 15316-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse ovary using 15316-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of A431 using 15316-1-AP
IP result of anti-THOC6 (IP:15316-1-AP, 4ug; Detection:15316-1-AP 1:300) with A431 cells lysate 2280 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary tumor using 15316-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15316-1-AP (THOC6 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	A431 cells, mouse ovary tissue, Neuro-2a cells
Positive IP detected in	A431 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

15316-1-AP targets THOC6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7561 Product name: Recombinant human THOC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC003118 Sequence: MTIFSQSVSPCGKFLAAGNNYGQIAIFSLSSALSSEAKEESKKPVVTFQAHDGPVYSMVSTDRHLLSAGDGEVKAWLWAEMLKKGCKELWRRQPPYRTSLEVPEINALLLVPKENSLILAGGDCQLHTMDLETGTFTRVLRGHTDYIHCLALRERSPEVLSGGEDGAVRLWDLRTAKEVQTIEVYKHEECSRPHNGRWIGCLATDSDWMVCGGGPALTLWHLRSSTPTTIFPIRAPQKHVTFYQDLILSAGQGRCVNQWQLSGELKAQVPGSSPGLLSLSLNQQPAAPECKVLTAAGNSCRVDVFTNLGYRAFSLSF Predict reactive species
Full Name	THO complex 6 homolog (Drosophila)
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC003118
Gene Symbol	THOC6
Gene ID (NCBI)	79228
RRID	AB_2240482
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86W42
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for THOC6 antibody 15316-1-AP	Download protocol
IP protocol for THOC6 antibody 15316-1-AP	Download protocol
WB protocol for THOC6 antibody 15316-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Viruses Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts. Authors - Toshie Sakuma KD Validated View Article
mouse	WB	Bone Res Nuclear farnesoid X receptor protects against bone loss by driving osteoblast differentiation through stabilizing RUNX2 Authors - Qi Dong KD Validated View Article
human, mouse	WB	Exp Cell Res THOC6 deficiency leads to cardiomyopathy by reducing myocardial contractile proteins in cardiomyocytes. Authors - Weihua Yuan KO Validated View Article
human	WB	Int J Biol Sci Targeting THOC2-Mediated mRNA Export Induces PARP Inhibitor Vulnerability in DNA Repair-Competent Hepatocellular Carcinoma. Authors - Xinli Li View Article