THOC6 Polyclonal antibody

• Featured Product
• KD/KO Validated

THOC6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15316-1-AP

Synonyms

fSAP35, THO complex subunit 6 homolog, THOC6, WDR58

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Validation Data Gallery

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  WB analysis using 15316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 15316-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15316-1-AP (THOC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 15316-1-AP

  IP result of anti-THOC6 (IP:15316-1-AP, 4ug; Detection:15316-1-AP 1:300) with A431 cells lysate 2280 ug.

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  IHC staining of human ovary tumor using 15316-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15316-1-AP (THOC6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, mouse ovary tissue, Neuro-2a cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

15316-1-AP targets THOC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THOC6 fusion protein Ag7561
• Full Name: THO complex 6 homolog (Drosophila)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC003118
• Gene Symbol: THOC6
• Gene ID (NCBI): 79228
• RRID: AB_2240482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Viruses; Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.; Authors - Toshie Sakuma ; KD Validated