THOP1 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 84319-3-RR (same clone as 84319-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84319-3-RR (THOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84319-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01191-3

  Sandwich ELISA standard curve of MP01191-3, Human THOP1 Recombinant Matched Antibody Pair - PBS only. 84319-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2268. 84319-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  Sample test of MP01191-3

  The mean THOP1 concentration was determined to be 154.80 ng/mL in THP-1 cell extract based on a 4.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP01191-3

  Cytometric bead array standard curve of MP01191-3, THOP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84319-2-PBS. Detection antibody: 84319-3-PBS. Standard: Ag2268. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 84319-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84319-3-RR against Human THOP1 were performed. The affinity constant is below 1 pM.

THOP1 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84319-3-PBS

Clone No.241607G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241607G8, EC:3.4.24.15, EP24.15, MEPD_HUMAN, MP78

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Product Information

84319-3-PBS targets THOP1 as part of a matched antibody pair:

MP01191-3: 84319-2-PBS capture and 84319-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2268

  Product name: Recombinant human THOP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 344-689 aa of BC000135

  Sequence: VEETRYCVDQNLLKEYFPVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARDAASGEVVGKFYLDLYPREGKYGHAACFGLQPGCLRQDGSRQIAIAAMVANFTKPTADAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLIESRQANTGLFNLRQIVLAKVDQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLNSKVGMDYRSCILRPGGSEDASAMLRRFLGRDPKQDAFLLSKGLQVGGCEPEPQVC

• Full Name: thimet oligopeptidase 1
• Calculated Molecular Weight: 689 aa, 79 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC000135
• Gene Symbol: THOP1
• Gene ID (NCBI): 7064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52888
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides