THUMPD1 Polyclonal antibody, PBS Only

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• THUMPD1 Polyclonal antibody, PBS Only (14921-1-PBS)

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  WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14921-1-AP (same clone as 14921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14921-1-AP (same clone as 14921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14921-1-AP (same clone as 14921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14921-1-AP (same clone as 14921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using THUMPD1 antibody (14921-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14921-1-PBS in a different storage buffer formulation.

THUMPD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

THUMP domain-containing protein 1, THUMPD 1

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Product Information

14921-1-PBS targets THUMPD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6739

  Product name: Recombinant human THUMPD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-353 aa of BC000448

  Sequence: MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGDDMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKLVHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNREEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSKQGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNENDFS

• Full Name: THUMP domain containing 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC000448
• Gene Symbol: THUMPD1
• Gene ID (NCBI): 55623
• RRID: AB_2271785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXG2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

THUMPD1, a THUMP domain-containing RNA-binding protein, partners with NAT10 to mediate N4-acetylcytidine (ac4C) modification, enhancing RNA stability, export, and translation. Dysregulated THUMPD1 promotes proliferation, migration, invasion, and EMT.