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TIM22 Polyclonal antibody, PBS Only (14927-1-PBS)

Filter: AllWBIPIHC

WB analysis using 14927-1-AP (same clone as 14927-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14927-1-AP (TIM22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.
IP experiment of mouse liver using 14927-1-AP (same clone as 14927-1-PBS)
IP result of anti-TIM22 (IP:14927-1-AP, 4ug; Detection:14927-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TIM22 Polyclonal antibody, PBS Only

Name: TIM22 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14927-1-PBS

TIM22 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14927-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14927-1-PBS targets TIM22 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6689 Product name: Recombinant human TIMM22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC002324 Sequence: MAAAAPNAGGSAPETAGSAEAPLQYSLLLQYLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAKEVLKDMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWKNSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR Predict reactive species
Full Name	translocase of inner mitochondrial membrane 22 homolog (yeast)
Calculated Molecular Weight	20 kDa
Observed Molecular Weight	20 kDa
GenBank Accession Number	BC002324
Gene Symbol	TIM22
Gene ID (NCBI)	29928
RRID	AB_11183050
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y584
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.