FcZero-rAb® APC Anti-Human TIM3 Rabbit Recombinant Antibody

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• FcZero-rAb® APC Anti-Human TIM3 Rabbit Recombinant Antibody (APC-FcA98115)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

TIM3 FcZero-rAb^® Recombinant Antibody for FC

Cat No. APC-FcA98115

Clone No.241422H1

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3

• 

  FC experiment of human PBMCs using APC-FcA98115

  1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with PE Anti-Human CD4 and 5 ul APC Anti-Human TIM3 Rabbit RecAb (APC-FcA98115, Clone: 241422H1). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using APC-FcA98115

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 5 ul APC Anti-Human TIM3 Rabbit RecAb (APC-FcA98115, Clone: 241422H1)(red) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human TIM3 Rabbit RecAb (APC-FcA98115, Clone: 241422H1) (dashed, black). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98115 targets TIM3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: hepatitis A virus cellular receptor 2
• Calculated Molecular Weight: 301 aa, 33 kDa
• GenBank Accession Number: BC020843
• Gene Symbol: TIM3
• Gene ID (NCBI): 84868
• RRID: AB_3746534
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDQ0
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	Clin Transl Med; TALDO1 promotes lipid metabolic reprogramming and immunosuppressive microenvironment remodelling in hepatocellular carcinoma; Authors - Fenglin Lv