TIMM44 Monoclonal antibody

TIMM44 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4G7D4

Cat no : 66149-1-Ig

Synonyms

MIMT44, TIM44, TIMM44



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66149-1-Ig is TIMM44 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TIMM44 fusion protein Ag21389
Full Name translocase of inner mitochondrial membrane 44 homolog (yeast)
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033628
Gene symbol TIMM44
Gene ID (NCBI) 10469
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIMM44 antibody 66149-1-IgDownload protocol
IHC protocol for TIMM44 antibody 66149-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 800CW Goat anti-Mouse1 h incubation at room temperatureLines on WB:1. BioRad Precision Plus Protein standards2. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ST-Hdh mouse striatal cell line
TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:66149-1-Ig)